- A spliced human DNA with insulin gene is identified
- Human insulin gene is cut using restriction enzymes
- Plasmid is cut with using restriction enzymes to provide fusion sites with insulin genes
- The cut human insulin gene is inserted into the cut plasmid using DNA ligase enzyme to form a recombinant plasmid
- The recombinant plasmid is introduced into a carrier bacterium to form a transgenic bacterium
- Transgenic bacterial clone multiples and produces insulin
- Insulin is extracted from the transgenic bacteria and purified
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